DBP and infection: First, we measured the diffusion coefficient of DBP by FRAP (Figure 3 and Supplementary Movies S7–S9) at three time-points post-infection: 16 hpi, which as described above corresponds to the early phase of viral replication in HAdV-infected HFF cells; 24 hpi, a time post-infection when an exponential increase in the accumulation of newly synthesized viral DNA molecules is observed; and 36 hpi, a late time-point when RCs have coalesced and viral progeny can be detected (see Figure 1A) (reviewed in [23]).