To evaluate the temporal aspects of PCD suppression by vIRA, vICA, vMIA and vIBO, we compared the parental virus to M45mutRHIM (inducer of RIPK3-dependent necroptosis), ∆M36 (inducer of CASP8-dependent apoptosis), and ∆M38.5/M41.1 viruses during infection of WT macrophages (Figure 3E and Figure S3C). This evidence concerns the gene CASP8 and infection.