To investigate the specificity of colorimetric LAMP assay with the newly designed RibB 5, RibB 16, and RibB 67 primers, genomic DNA of 56 bacterial strains (i.e., 38 Burkholderia strains (20 BCC strains and 18 non-BCC strains) and 18 non-Burkholderia bacterial species) were extracted individually and about 10 ng/μL were used for the LAMP reaction. This evidence concerns the gene RIT1 and skin basal cell carcinoma.