We found that Eph receptors seemingly play a role in CCA biology; EPHA1-4, EPHB3 were highly expressed whereas, EPHA7, EPHA8, EPHA10, EPHB1, EPHB2, EPHB4, and EPHB6 had a lower expression in CCA tumors compared to normal tissues. This evidence concerns the gene EPHB6 and cholangiocarcinoma.