The concentration of zinc in the tissues and blood of osteosarcoma patients has been found significantly reduced with respect to healthy subjects [169]; further studies reported that zinc administration showed an antineoplastic effect, causing a drop in proliferation and invasion rates and leading to caspase-3 and- 9-related apoptotic cell death in U-2OS osteosarcoma cells, activating the Wnt/β-catenin pathway by increasing the levels of Wnt3 and β-catenin [168]. This evidence concerns the gene CASP3 and osteosarcoma.