Lysis of the cells with 1% (v/v) Triton X-100 revealed variable LDH levels within the different cell lines with maximum values for the colon carcinoma cell lines HCT-116 and HCT-116/p53(−/−), as well as for A498 (renal cell carcinoma) and MiaPaCa-2 (pancreatic carcinoma), and minimal values for Capan-2 (pancreatic carcinoma) and Hep3B (hepatocellular carcinoma). Here, TP53 is linked to hepatocellular carcinoma.