To illustrate whether rutin-induced cytotoxicity in SiHa cancer cells was associated with the activation of caspases (Caspase-3 and -9), SiHa cells were pretreated with 50 μm of Caspase-3 and -9 inhibitors (Z-DEVD-FMK and Z-LEHD-FMK) for 2 h and then treated with selective doses of rutin for 24 h (Figure 5C,D). Here, CASP3 is linked to cancer.