These results suggest that TLR3 is mainly implicated in cytokine secretion control and type 1 IFN expression 24 h post-infection; TLR7 controls the expression of type 1 IFN, IFN-γ, and IFN-λ3 expression in the late phases of SARS-CoV-2 infection. This evidence concerns the gene TLR3 and infection.