Furthermore, although the expression of the cytokines IFN-γ and perforin in CD8+ T cells was upregulated after RFA and cryo-thermal therapy compared to tumor-bearing mice, the expression levels of IFN-γ and Granzyme B in CD8+ T cells after cryo-thermal therapy were much lower than those in the RFA group (Figure S1). The gene discussed is PRF1; the disease is neoplasm.