We have previously described the generation of two stable lines derived from parental human colorectal adenocarcinoma Colo-320 cells: Colo-320/CD9, expressing high levels of cell surface CD9 after stable transfection; and Colo-320/ADAM17-KO, lacking expression of the metalloproteinase ADAM17/TACE after specific CRISPR/Cas9 knock-out of this enzyme [14] (see also supplementary material). Here, CD9 is linked to colorectal adenocarcinoma.