In sum, our data unveil that the interaction of cellular integrin α5β1 with ADAM17 exposed on the surface of exosomes is negatively regulated by CD9 and plays a role in EV adhesion and uptake by CRC cancer cells and PMCs, suggesting that these molecules could be relevant therapeutic targets to inhibit CRC peritoneal carcinomatosis. This evidence concerns the gene CD9 and colorectal carcinoma.