Using Snail1 overexpression models of melanoma cells, it was suggested that production of TGF-β and thrombospondin-1 (TSP1) appears to generate immunosuppressive CD4 + Foxp3+ T cells (Tregs) and non-responsiveness of CD8+ T cells, resulting in enhanced tumor metastasis in various organs of the B16-F10 mouse model [31]. Here, CD4 is linked to melanoma.