TF and neoplasm: While Twist1 activation (an EMT-TF) is required in promoting EMT and CTCs, turning off Twist1 at distant sites allows MET and is essential for disseminated tumor cells to proliferate and form metastases [12]; however, alternative modes of dissemination such as collective or cluster-based migration and invasion can exist, whereby cells do not need not to shed cell–cell adhesion completely while having gained the traits of migration and invasion [13].