The Caco-2 cells are not characterised as metastatic cells, they show a well differentiated phenotype, HCT-116 cells are able to form tumours when inoculated in BALB/Nude mice, while DLD-1 show positivity to vimentin staining (Supplementary Figure S3) representing the most aggressive colon cancer cells, able to form distant metastasis. Here, VIM is linked to colonic neoplasm.