THP-1 and hTERT-HSC were identified by CD68 and vimentin staining, confirming successful incorporation of all three cells lines, as previously reported by Prestigiacomo et al. [49] TGF-β1 is a key cytokine in liver fibrosis progression and is described as a key event (KE) in the AOP for liver fibrosis. The gene discussed is TGFB1; the disease is Hepatic fibrosis.