MAPT and neurodegenerative disease: Generally, our findings reveal the role of tau N-terminal inserts in tau LLPS in live neuronal cell lines, and in the context of the previously proposed hypothesis that tau droplet (spherical droplet-shaped accumulations) formation can lead to tau aggregation [7], tau N-terminal inserts may hold promise as a novel therapeutic target to intervene in tau pathological aggregation in neurodegenerative diseases.