CCL2 and melanoma: Our findings indicate that B16–BL6/Zs green murine melanoma cells spontaneously secrete the chemokine CCL2 into the culture media (supernatants from cultures without gabapentin), contrary to that reported for B16-F10—another variant cell line of B16 murine melanoma that does not release CCL2 (59.44 ± 10.11 pg/mL at 24h, 255.00 ± 29.41 pg/mL at 96 h, and 588.03 ± 85.20 at 72 h after washout) [31,32].