AIFM1 and brain ischemia: Both MCAO/R and OGD/R induced the activation of PARP-1 and the increase in poly (ADP-ribosylation) in neurons and led to nuclear translocation of AIF, while 14,15-EET pre-treatment inhibited the activation of PARP-1 and reduced the nuclear translocation of AIF (Figure 3, Figure 4 and Figure 5), suggesting that 14,15-EET pre-treatment can inhibit neuronal parthanatos induced by cerebral ischemia and reperfusion (Figure 3).