HMOX1 and brain ischemia: qPCR results showed that the pretreatment of 14,15-EET via tail vein injection increased the transcription of certain antioxidant genes such as glutathione peroxidase (GSH- Px), heme oxygenase-1 (HO-1) and superoxide dismutase (SOD), while catalase (CAT) gene expression showed no increase, suggesting that 14,15-EET can inhibit the production of reactive oxygen species induced by cerebral ischemia and reperfusion by upregulating the expression of antioxidant genes such as GSH-Px, HO-1 and SOD (Figure 7).