Here, we report a study, in which PDT, combined with the intratumoral administration of PLGA NPs (loaded with poly(I:C), R848, and MIP3α), was analyzed for its therapeutic efficacy, compared to modality alone, in three murine cancer models: MC38 (colon adenocarcinoma model), CT26 (colon cancer carcinoma), and TC-1 (lung epithelial tumor expressing human papillomavirus (HPV)16 E6 and E7 oncoproteins). This evidence concerns the gene CCL20 and cancer.