The instillation of CA-TRA, nTRA, and nTRA-F127 did not attenuate the EB exudation, NO levels, or TNF-α levels in the conjunctiva; however, the EB exudation in conjunctivitis rats was significantly prevented by the instillation of combination-ISGs incorporating Tra-NPs (nTRA-MC/F127, nTRA-F68/F127, and nTRA-Car/F127). The gene discussed is TNF; the disease is conjunctivitis.