Moreover, after intravenous immunization in C57BL/6 mice, the following facilitated in vivo immune responses were observed, as compared with any single delivery of a cargo agonist: (1) downregulation of DC-secreted immunosuppressive IL-10, (2) significant reduction in tumor-infiltrating regulatory T cells, (3) enhanced activation of CTLs, and (4) effective inhibition of tumor growth. Here, IL10 is linked to neoplasm.