There was no statistical difference in cell viability up to 100 nM, whereas 500 nM and above of calcitriol treatment resulted in cell death in both HT29 and SW480 human colorectal cancer cells, as shown in Figure 1A. Colorectal cancer cells often exhibit active Wnt/β-catenin signaling, and previous literature reported that calcitriol at a concentration of 100 nM for 48 h appears to upregulate the expression of E-cadherin and modulate β-catenin signaling in SW480 cells [19]. Here, CDH1 is linked to colorectal cancer.