To further verify whether PI3K/Akt/mTOR signaling pathway is involved in the process of 4-HD inducing apoptosis and cell cycle arrest of hepatoma cells, as shown in Figure 5A,B, the proportion of apoptosis in the 4-HD + LY294002 treatment group was increased, compared with the control group (p < 0.001). The gene discussed is AKT1; the disease is hepatocellular carcinoma.