The reduction of IFN-γ in the supernatant of spleen leukocytes observed in the CanL group after supplementation with 1,25(OH)2VD3 confirmed the anti-inflammatory role of vitamin D, which was also observed in experimental leishmaniasis [54,55] and in other infectious parasitic [56,57] and autoimmune diseases [58]. This evidence concerns the gene IFNG and autoimmune disease.