Interestingly, ACLY mRNA expression was reported to increase in Huh7 hepatocellular carcinoma cells with SIRT6 overexpression and decrease with miR122 which is an antagonist of SIRT6 [54]; by contrast, our findings here indicate that SIRT6 deficiency increases ACLY expression in U2OS cells at the level of mRNA and protein (Figure 1 and Figure S1). Here, SIRT6 is linked to hepatocellular carcinoma.