Specifically, the variant allelic frequencies (VAFs) for the 4-nt TCTG insertion/duplication and the new 8-nt deletion were 16% and 20.5%, respectively, in keeping with the about 40% AML blasts representation in the analyzed patient’s sample (considering that NPM1 mutation are heterozygous) (Table 1). Here, NPM1 is linked to acute myeloid leukemia.