Instead, all cases with mutation in exon 12, but one, were recognized by the anti-NPM1 mutant antibody at WB confirming the value of this methodological approach in identifying at least the great majority of NPM1 mutant proteins in AML samples and in predicting the underlying mutation in NPM1 exon 12 [20] (Discrepancy 2, Figure 1). Here, NPM1 is linked to acute myeloid leukemia.