The analysis of the expression levels of the genes involved in important metabolic pathways for glioma cells revealed that, in order to meet the needs of the highly proliferative cells of gliomas, they undergo metabolic remodeling, which can be summarized as an increase in glucose consumption for a higher production of glycolytic ATP and lactate as the final product, where the overexpression of genes involved in glycolysis such as HK1, PFKM, GAPDH, and LDHAL6A was observed. This evidence concerns the gene PFKM and glioma.