Knockdown of NRF2 with RNAi resulted in a significant decrease in NRF2 signalling in three CRC cell lines as assessed by validated NRF2 transcriptional targets, NQO1 and HMOX1 [26], as surrogates of pathway perturbation [27] (Supplementary Figure S1a–c). This evidence concerns the gene NFE2L2 and colorectal carcinoma.