INS and type 2 diabetes mellitus: For this reason, we documented prospectively the changes of TBARS, ABTS, TAC, RP, before and after 4 and 12 months of treatment in four parallel groups of T2DM treated with insulin, GLP-1RA, SGLT-2i and the combination GLP-1RA and SGLT-2i as a second step treatment to metformin for one year correspondingly.