Specifically, the cytotoxic effect of these compounds could be tested in vivo in 22Rv1 xenografts implanted in nude mice [64], as a model of PCa with hyperactivation of p-ERK, or in LNCaP or PC-3 xenografted mice, as these cells are PTEN-null and the AKT pathway is highly activated [65]. This evidence concerns the gene MAPK1 and posterior cortical atrophy.