The estimated risk of familial ESCC carrying LOF RAD50 variants (7/2088, 0.34%) was moderately increased compared to Henan sporadic ESCC cases and controls (8/4490, 0.18%), although this was not statistically significant (OR 1.88, p = 0.33). The gene discussed is RAD50; the disease is esophageal squamous cell carcinoma.