To perform these analyses, we used the tools and statistics of cBioportal (https://www.cbioportal.org, accessed on 3 March 2021) and defined two groups of samples in the prostate and ovary cancer studies of the TCGA Pan Cancer Atlas; one group composed of samples with HMGB1 mRNA levels lower than mean − 1.5 standard deviation (HMGB1-low) and a second group expressing mRNA levels higher than mean + 1.5 standard deviation (HMGB1-high) (Figure 5A). Here, HMGB1 is linked to cancer.