Zakikhani et al. revealed that treatment with 5~20 mM metformin more effectively reduced cell proliferation of breast (MCF-7), ovary (SKOV3 and OVACR-3), and prostate (PC-3) cancer cells through up-regulation of AMPK and down-regulation of mTOR and p70S6K [40]. This evidence concerns the gene RPS6KB1 and cancer.