To study the early events in the melanoma TME that shape the TAM tumor-promoting profile, we proposed to build a 3D in vitro culture system using the Yale University Melanoma Model (YUMM) 1.7, which has genetic changes common to human melanomas, including BRAFV600E, Pten−/−, and Cdkn2−/− [35,36]. Here, PTEN is linked to melanoma.