CDH1 and esophageal squamous cell carcinoma: Furthermore, double immunofluorescence using anti-E-cadherin and anti-β-catenin antibodies, to confirm the expression and localization of E-cadherin and β-catenin in the ESCC cell lines treated with siNC or siMT2A, revealed an enhancement in the co-localization of E-cadherin and β-catenin in both the ESCC cells transfected with siMT2A compared with that in cells transfected with siNC (Figure 5J).