Although the flow cytometry results show the unstuck cell cycles when co-treated with Cur and 5-FU, the Western bolt results show the dramatic synergistic effect of Cur and 5-FU on the inhibition of p-Rb, cyclins, and CDKs, thus, we assume that Cur can reinforce the disorders induced by 5-FU on the cell cycle in CRC cells. The gene discussed is RB1; the disease is colorectal carcinoma.