Jiang et al. have reported that bufalin exerts its antiproliferative effect on A459 non-small cell lung cancer (NSCLC) cells in a time-dependent manner [53], where bufalin reduced the cellular viability of A549 cells by enhancing the expression of p53 and p21 (WAF1/CIP1) genes. This evidence concerns the gene CDKN1A and non-small cell lung carcinoma.