To validate the expression of PITPNM1 in different breast cancer cell lines and a non-tumorigenic immortalized breast epithelial cell, the mRNA levels of PITPNM1 were determined in nine cell lines including HER2 over-expression, TNBC, luminal subtypes, and non-tumorigenic MCF-10A (Figure 3B). This evidence concerns the gene ERBB2 and breast carcinoma.