Furthermore, Luo et al. reported that docosahexaenoic acid (DHA; C22; n-3) improved NAFLD by activating Sirt1 in a high-fat diet-induced NAFLD mouse model and prevented the accumulation of palmitic acid-induced lipid droplets, the decrease of fatty acid oxidation and the reduction of SIRT1 level in HepG2 cells [116]. Here, SIRT1 is linked to metabolic dysfunction-associated steatotic liver disease.