Based on the above mentioned literature and integrating it with the study statistical results, we can hypothesize that the development of NASH led to upregulation of lncRNA lnc-SPARCL1-1:2, resulting in upregulation of miR-6881-5p miRNA with a concomitant increase in the levels of HSPD1 mRNA, MMP14 mRNA and ITGB1 mRNA, confirming our bioinformatics findings and validating the role of the RNA panel in predication and early detection of NASH (Figure 5). This evidence concerns the gene ITGB1 and metabolic dysfunction-associated steatohepatitis.