Of note, GLI3 overexpression in colorectal cancer cells has been implicated in enhanced colony formation, proliferation, and invasion by upregulating the expression of EMT factors (TWIST1, ZEB1, VIM, ZEB2, and CDH2) via positive regulation of ERK1/2 cascade, increasing the expression of adherence-related genes (ITGA4, GDF15, and NXPH4), and downregulating p53 levels. This evidence concerns the gene GLI3 and colorectal cancer.