In this study, we successfully developed a novel DPPC-Fe3O4-plasmid complex to deliver liver-specific albumin promoter-driven BDD-hFVIII gene expression for more than two weeks in a mouse model of hemophilia A. The binding ability of circular DNA was confirmed to be more stable than that of linear DNA when combined with DPPC-Fe3O4 nanoparticles. Here, ALB is linked to hemophilia A.