Accordingly, in this study, in order to investigate the applications and potential role of CDCA in islet transplantation, healthy islets were harvested from mice, encapsulated in CDCA microcapsules, and surgically transplanted into the epididymal tissues in pelvic region of 6 syngeneic diabetic mice (injected with alloxan to induce T1D, confirmed with blood glucose >16 mM in two consecutive measurements over two days, and absence insulin in blood). The gene discussed is INS; the disease is type 1 diabetes mellitus.