PDCD1 and neoplasm: Again, to stamp the selective function of immunosuppressive cells in inhibiting anti-PD-1 effect, they specifically depleted T-regs (anti-CD25) and PMN-MDSCs cells in the NTC control mice, resulting in delayed tumor progression as compared to the ALKBH5−/− model (due to already fewer T-reg numbers), confirming the immunosuppressive function of T-regs (induced by MDSCs) in impairing the efficacy of anti-PD-1 antibody by inhibiting CD8+T-cells effector functions through decreasing DC-differentiation (CD45+Ly6C-MHC-II+CD24hiF4/80lo) markers [165].