To this end, Li and colleagues used B16 (mouse melanoma) and CT26 (colorectal carcinoma)-induced TBM model, and selectively depleted ALKBH5 and/or FTO (CRISPR/Cas9-mediated silencing) in B16 and CT26 cell lines respectively, and injected subcutaneously into wild-type C57BL/6 and BALB/c mice to create tumor, followed by 1-day prior vaccination with irradiated B16 cells secreting GM-CSF ‘GVAX’ to induce sufficient antitumor T-cell response, and finally anti-PD-1 antibody treatment was given to check its efficacy. The gene discussed is FTO; the disease is melanoma.