Furthermore, reducing SLFN5 in these cells activated the β-catenin signaling pathway, enhancing Metallothionein 1 (MT1)-MMP expression and increasing migration and invasion of A549 lung adenocarcinoma cells in vitro and even in vivo, when SLFN5 knockout A549 cells were injected in chorioallantoic membrane of the chick embryo [77]. The gene discussed is SLFN5; the disease is lung adenocarcinoma.