In the case of osteosarcoma specific themes, Fig 8 shows that SPARK, LUM, SERPINH1, MRC2, and PCOLCE are associated with “collagen binding,” COL5A2, LUM, COL6A3, COL12A1, TNC, and PCOLCE are associated with “extracellular matrix,” COL5A2, COL12A1, CDH11, and ALPL are associated with skeletal system development, COL5A2, SPARC, and CDH11 are associated with “ossification,” and SPARC, CALU, CDH11, and FKBP10 are associated with “calcium ion binding.” The module genes have average CTD number of references = 71 associating them with osteosarcoma and an average CTD inference score = 17. Here, TNC is linked to osteosarcoma.