By means of a one-way analysis of variance (ANOVA) test, we identified that 1) the average lifespan of cytotoxic CD8+ T cells (CTLs) within the pancreatic islets shown in Fig 6A; 2) the initial number of damaged beta cells when the simulation step equals 0 shown in Fig 6B; 3) time interval of dendritic cells (DCs) movement in islets shown in Fig 6C; 4) the maximum number of naïve CD8+ T cells binding to DCs shown in Fig 6D; 5) recruitment rate of DCs within the pancreas shown in Fig 6E are the five sensitive parameters during T1D progression. This evidence concerns the gene CD8A and type 1 diabetes mellitus.