To assess the neuroprotective potential of astrocyte-derived miR-20a-3p in the context of stroke, a viral construct (rAAV5-TetOn-GFAP-miR-20a-3p-mCherry, SignaGen) under the control of a TetOn system with a GFAP promoter and conjugated with a mCherry reporter (Supplementary Figure 3a) and a control vector (Supplementary Figure 3b) was synthesized and injected into the left hemisphere of middle-aged female rat brains. The gene discussed is GFAP; the disease is stroke disorder.