In order to detect SNVs from CHD patients, a total of 252 genes important for cardiac development were selected for detection of single-nucleotide polymorphisms (SNPs), including 149 genes of signaling transduction (WNT, TGFβ, Notch, ERBB, FGF, and Cilia-Hedgehog), 40 genes of transcriptional factors, 46 genes related to “Folate and one carbon metabolism,” and 17 genes of structural proteins. The gene discussed is TGFB1; the disease is coronary artery disorder.