PLS1 and hearing loss disorder: Consistent with these GO analysis results, Up-trend DEGs include many genes related hearing loss such as MYO7A, CLRN1, WHRN, FSCN2, PCDH15, PLS1, CIB2, USH1C, THRB, and PTPRQ. On the other hand, in Down-trend DEGs expressed higher levels at the base, enriched GO terms include broad developmental and biological processes such as system development, multicellular organismal processes, and signal transduction.