In terms of mechanisms, both immunofluorescence assay and western blotting assay validated that ursolic acid treatment significantly attenuated the expression levels of glycolysis-associated proteins (LDH-A and c-Myc) whereas increasing SP1 and Cav-1 expression levels in mammary tumor tissues (Figures 6E, F and Supplementary Figure 11). This evidence concerns the gene CAV1 and breast cancer.