A negative association between ASF1B expression levels and DNA methylation were observed in ACC, Bladder Urothelial Carcinoma (BLCA), CESC, COAD, LGG, LIHC, LUSC, MESO, PAAD, READ, PRAD, Sarcoma (SARC), SKCM, STAD, Testicular Germ Cell Tumors (TGCT), Thyroid carcinoma (THCA), and UCEC (P<0.05) (Figure 3A). The gene discussed is ASF1B; the disease is thyroid gland carcinoma.